Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources.

نویسندگان

  • T Adam
  • G S Gassmann
  • C Rasiah
  • U B Göbel
چکیده

A total of 17 B. burgdorferi isolates from various sources were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, restriction enzyme analysis, Southern hybridization with probes complementary to unique regions of evolutionarily conserved genes (16S rRNA and fla), and direct sequencing of in vitro polymerase chain reaction-amplified fragments of the 16S rRNA gene. Three groups were distinguished on the basis of phenotypic and genotypic traits, the latter traced to the nucleotide sequence level.

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عنوان ژورنال:
  • Infection and immunity

دوره 59 8  شماره 

صفحات  -

تاریخ انتشار 1991